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KMID : 0545120070170050858
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Journal of Microbiology and Biotechnology
2007 Volume.17 No. 5 p.858 ~ p.864
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Antibacterial Activity of Sophoraflavanone G Isolated from the Roots of Sophora flavescens
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Cha Jeong-Dan
Jeong Mi-Ran
Jeong Seung-Il
Lee Kyung-Yeol
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Abstract
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This study investigated the antibacterial activities of sophoraflavanone G from Sophora flavescens in combination with two antimicrobial agents against oral bacteria. The combined effect of sophoraflavanone G and the antimicrobial agents was evaluated using the checkerboard method to obtain a fractional inhibitory concentration (FIC) index. The sophoraflavanone G+ampicillin (AM) combination was found to have a synergistic effect against S. mutans, S. sanguinis, S. sobrinus, S. gordonii, A. actinomycetemcomitans, F. nucleatum, P. intermedia, and P. gingivalis, whereas the sophoraflavanone G+gentamicin (GM) combination had a synergistic effect against S. sanguinis, S. criceti, S. anginosus, A. actinomycetemcomitans, F. nucleatum, P. intermedia, and P. gingivalis. Neither combination exhibited any antagonistic interactions (FIC index>4). In particular, the MICs/MBCs for all the bacteria were reduced to one-half ~ one-sixteenth as a result of the drug combinations. A synergistic interaction was also confirmed by time-kill studies for nine bacteria where the checkerboard suggested synergy. Thus, a strong bactericidal effect was exerted through the drug combinations, plus in vitro data suggested that sophoraflavanone G combined with other antibiotics may be microbiologically beneficial rather than antagonistic.
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KEYWORD
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Sophora flavescens, sophoraflavanone G, antibacterial activity, checkerboard method, time-kill method, synergic effect
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